I am performing ChIP-assay and due to some reason I have to stop my assay. Can I store my sample at -20 degree after addition of 5 M NaCl and incubating at 65 degree for 4 hrs? Currently I am at this step.
Hello everyone, I am performing western blotting with the tissue lysate from mouse muscle tissue and after quantifying my protein concentration I am loading 20ug of protein in each well but I am...
07 August 2019 10,075 11 View
Hi, I am trying to perform IP with HSF1 antibody from liver tissue but no success so far. I prepared my tissue lysate in RIPA buffer and I am using 1mg protein for IP and bead used is protein A/G...
01 February 2019 8,633 8 View
Hi, we are trying to isolate DNA-free RNA from neural stem cell for RNA sequencing but our RNA is DNA contaminated even digesting twice with DNae. Could you suggest some kit or method which really...
02 March 2017 7,432 12 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View