I'm developing an assay to determine sensitivity to a hypomethylation chemotherapy agent. Preliminary cytotoxicity assays were conducted to determine dosages that yield the optimum viable cultured cell count to hypomethylation ratio via Trypan Blue & Cell TitierGLO assays. In the attached file you can see i have graphed the data with error bars. Is it necessary to conduct any more analyses via using methods such as ANOVAs?
ANOVA only determines if there is statistical significance between any two groups within your dataset. You would need to perform post-hoc test such as Bonferroni or Tukey's (it depends on the type of comparison you are trying to show). However, if you trying to compare to your non-treated group, a simple T-test would suffice.
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