I'm working on quinoa saponins. I want to examine the accuracy of the method as well as the recovery
rate of extraction so im gonna perform a standard addition test.
Is alpha-hederin ok to use as an addition material? Any idea Which standard should i use: analytical standard (purity 90 % HPLC) or primary pharmaceutical reference standard (certificate in each package)?
Dear Asta, any standard with declared assay and purity is fit for the purpose provided you do not need to use some sort of certified material because the results of your work are intended to be used in regulated environment, such as GMP, GLP or HACCP. Then use the product recognised as a reference material in the respective field. As for the purpose of the study, I understand you intend to use standard addition to check the recovery rate of extraction. While you can certainly use standard addition to check the extraction from HOMOGENEOUS sample, the method is not suitable for checking the extraction yield from non homogeneous samples such as grains or flour. You may wish to use a reference material of the quinoa grains with certified amount of saponins (should one exist), alternatively you might use repeated extraction to calculate the yield.
For HPLC you need at least a 99% reference standard, which has already been identified and qualified (CoA/SigmaAldrich?).
Otherwise what is the other 10%? Which is the right peak?
In addition two nice comments above, you can read AOAC appendix k page 5 regarding purity of the standard.
http://www.eoma.aoac.org/app_k.pdf
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