Dear all,
Im running an Inject & recover experiment on Biacore T200. I have a couple of different analytes to inject on sensor and then recover for mass spec analysis, each analyte to separate eppendorf. I can't figure out to which rack position does the "recovered" sample go to. Does anyone know how to check where the recovered sample is being released?
Thanks!
Dear Filip, it will be easier to contact your application scientist for technical support ( [email protected])
have you tried this assay? the BIacore software did not show where the eluates would be deposited?
I need to perform a similar assay in BIacore T200, I don't know how to recover the samples yet.
According to the BIacore T200 manual:
A small volume (approximately 2 μL) of Recovery solution, separated from the surrounding buffer by air segments, is injected into the flow cells. The flow is stopped for the specified Incubation time while the recovery solution is in contact with the sensor surface, to allow the bound analyte to dissociate into the recovery solution.
The flow direction over the sensor surface is reversed and the recovery solution containing recovered analyte is deposited in the target position where it mixes passively with the deposition solution.
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