I have collected some populations of brown planthopper (Nilaparvata lugens). I'd like to identify its biotype with molecular methods by using a specific primer. Does anyone have this type of experience?
Dear candra..
I don't have research experience with plants. but i am having t similar experience in insects-primer designing methods. My kind suggessions is that, u can find the specific primer of ur collected species with the help of NCBI and primer 3 output and spidy analysis.By giving the Nilaparvata sp., name in to the specific marker like (EST,RAPD,SSR) u ll get ur CDNA sequenceS from that sequences u go to Primer 3 online tool package free ver. software will help u to find the exact primer sequences of ur sp. and spiedy analysis will help u to find the matches,exon and intron sequences.
Best of luck..
Dear Candra Putra,
We are now trying to identify QTL for virulence or biotype of brown planthopper, after the genetic linkage map of the BPH has been developed (Jairin et al., 2013). Further study, we found some loci (in LG10 and LG14) that might be associated with the virulence or biotype in some BPH populations. However, the data is not yet available for public.
There are several studies that tried to differentiate the BPH populations using the mitochondrial cytochrome oxidase-I (COXI) gene (Mun et al., 1999; Naeemuliah et al., 2009) or SSR (Jing et al., 2011) but not for differentiate the biotype. Up to recently, I thought there is no specific molecular marker for BPH biotype has been developed. Many laboratories in Asia are now trying to develop the markers. I hope that the study will be easier when the whole genome sequencing data of BPH are available (within this year).
Jirapong
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