Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

Change of First name?

In some publications you refer Deivid Chica (with Barranco) but the true first name is Maria Dolores Chica , in spanish Ma. Dolores Chica. Before I answer yes is an author Deivid Chica, but...

18 February 2021 5,004 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Participation in questionnaire for PhD Thesis, any English, Chinese or Spanish speakers?

Hello! I am looking for Spanish, English and Chinese native speakers to participate in my final survey for my PhD thesis. https://claudiomastrangelo4.wixsite.com/phdlinguist1/research This is...

01 February 2021 4,177 10 View

What's the main sources for a study of "ethics" in the 1500-1620 period?

I would like experts to provide me with the philosophers of the 1500-1620 period who discussed "ethics" in all or parts of their career. Erasmus, Montaigne, Castiglione, Bruno, Sidney and their...

04 January 2021 7,054 3 View

What is a variabel to analyze empirically or attest a theory of Malthus (Economic Major)?

I have a project to evidence Thomas Robert Malthus theory about a principal of population (Malthusian Spectre/Malthusian Trap). I want to attest, his theory about a relationship of population and...

02 January 2021 8,146 3 View

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01 January 2021 5,831 1 View

Do you know anything about Elena Bokus, the early 20th Century Architect / Architectural Designer?

The available research of early 20th century history of architecture in North Macedonia, suggests that probably the first female architect / architectural designer was Elena Bokus (born in 1893...

23 December 2020 2,039 2 View

What is the best Quality of Life questionnaire for chronic patients?

Hello! I am looking for a SHORT questionnaire/scale to measure Quality of Life in chronic patients (e.g. fibromyalgia, or multiple sclerosis). Ideally, validated for English and Spanish speakers....

22 December 2020 9,962 4 View