Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

After staining a gel with coomassie can I stain the same gel with fluorescent dye?

I want to know if it is possible to be done the inverse to view / verify other bands that not look in staining a gel with coomassie.

01 February 2021 8,647 5 View

What a 50% positive predictive value mean?

I want to explain a threshold of 50% PPV to describe how some assays perform when the prevalence of disease is high or low

07 October 2020 8,657 6 View

Author who is not actually an author of some of my publications?

I came out to realize that a person who is enlisted as an author of some publications that I am the REAL AUTHOR, is marked instead of me. How do I change that? Example: Standardized sampling...

21 September 2020 378 7 View

How to increase the short circuit current density in a perovskite solar cell?

I'm optimizing the procedure for making inverted PSCs: ITO / PTAA(8nm) / CsMAFA(320nm) / PCBM(60nm) / BCP(9nm) / Ag(100nm). The cells look very good, I have a very good FF (0.70) and Voc (1.0V),...

16 September 2020 6,286 8 View

How can I identify and catalog the Rainforest fungus?

We have a big area of Rainforest where we can see a big variety of fungos growing around. I'd like to catalog them using a simple methodology. We do not have equipment for that despite a...

16 March 2020 9,034 3 View

Sensors and Systems for IIoT?

13 March 2020 7,295 3 View

Does someone know the cause of stains appearing on n-i-p Perovskite Solar Sells?

I did the procedure described by saliba ( How to Make over 20% Efficient Perovskite Solar Cells in Reg... ) to make n-i-p PSCs. However, after perovskite deposition stains appeared in the cell...

29 October 2019 8,012 3 View

How to process small samples for histology?

Hi, I have very small samples of about 1x2mm (dental pulp tissue) and I need to process them for histology (dehydration, parafine inclusion, stainning with HE, etc). The problem is that the...

18 September 2019 1,180 4 View

What is the best protocol for evaluate TMR-peptide?

I apologize if my question seems to naive, but I would like to do a protocol to determine bio-distribution of a peptide coupled to TMR in an animal model however, I am having some difficulties in...

07 July 2019 5,664 2 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View