For the determination of pyruvate dehydrogenase, absorption coefficient of 2,6-Dichlorophenolindophenol is required. If anybody know this value please share with me?
See in this link. It might be helpful
https://answers.yahoo.com/question/index?qid=20130128014208AAvdlql
https://www.researchgate.net/publication/9417068_The_molar_extinction_coefficient_of_26-dichlorophenol_indophenol
Article The molar extinction coefficient of 2,6-dichlorophenol Indophenol
YOU SHOULD SEND YOURSELF a mail to the AUTHORS via RESEARCHGATE ans ask for the publication!
According to the excellent answers of the answerers, I send you the required article as pdf.
But what do you mean with universal formula????
thank u Arnab but i want universal formula for determining extinction coefficient,,,,,,
JRG
Dear Kanu,
Please more infos for :
-You write : "For the determination of pyruvate dehydrogenase...." Can you reformulate your question and write :
pyruvate dehydrogenase e.g. activity of ...... in human or animal p.e.!!!
Harry and I are waiting for the meaning of your sentence. Please see the following link:
-This link and the other links found at this site can be useful for the " Search of your universal formula"
https://www.youtube.com/watch?v=XhZQgC1Z6_8
Spectrocopical Hints:
According to the BBA pdf please note the following hints:
- If you buy DCPIP , you obtain it as sodium salt or sodium salt with n. moles water.
Amstrong dried it over P2O5 to constant weight in order to obtain the anhydrous salt.
For the concentration you need it for a correct molecular weight.
- Stability (see Amstrong) in a certain pH range.
JRG
DETERMINATION OF PYRUVATE DEHYDROGENASE FROM MAMMALIAN (MICE) HEPATIC TISSUE , FOR THAT REASON EXTINCTION COEFFICIENT OF DCPIP AT PH 7.0 REQUIRED ..AND I GET DATA FROM THE PDF FILE [BBA] .... ACTUALLY DCPIP IS USED AS ELECTRON ACCEPT ER THERE IS ANOTHER PROCEDURE FOR DETERMINING THE ACTIVITY OF PYRUVATE DEHYDROGENASE BY USING FERROCYANIDE BUT IT IS NOT VERY MUCH SENSITIVE ... AND AT LAST MY HEARTILY THANKS to Jean Rene Grezes Sir & Harry ten Brink Sir for solving this problem.
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