Perhaps the following recent articles on Dispersive Liquid-Liquid Microextraction (DLLME) using some of the organic phases, extracting agents, and chelating compounds you have mentioned might be helpful, or at least of interest, to you:

1) Dispersive Liquid-Liquid Microextraction of Copper Ions as Neocuprione Complex in Environmental Aqueous Samples – SHARIATI and GOLSHEKAN, 2011

http://acta.chem-soc.si/58/58-2-311.pdf

2) Speciation of Fe(II) and Fe(III) by using Dispersive Liquid-Liquid Microextraction and Flame Atomic Absorption Spectrometry – BAHAR and ZAKERIAN, 2012

http://www.jbcs.sbq.org.br/online/fpapers/11725AR.pdf

Thanks for your answer and help :) ! cheers

You are quite welcome. Good luck!

Both metals are in aqueous medium? If your response is positive, you can just add ammonium hydroxide in excess. Copper will form a blue complex and iron will be precipitated as iron hydroxide.

Thanks for your answer. I finally do a L-L extraction with choloroform and ditizone as extracting agent. Cheers