What is the average yield of each coupling and deprotecting sequence taken as one assuming that the final cleavage step from the solid support was quantitative?
Dear Amirah Aldawsari ,
Assuming that every step occurs at the same yield then a rough estimate would mean: a yield of around 98,6% (see calculation1).
Not entirely sure what you mean by “taken as one” but I guess you mean 124 steps (instead of 248), this would render a yield of 97,2% (see calculation2).
See also: https://blog.gyrosproteintechnologies.com/purepep/peptide-purity-yeild-spps
Hope this answers your question somewhat.
Best regards.
Hi, Amirah Aldawsari Using Prof. Rob Keller´s calculations you can realize that the synthesis was very efficient, and not as "bad" as you might be thinking. If you come from the organic synthesis field, then it is frustrating to get these "low" yields. However, you have to remember that during the SPPS lot, a lot! of steps are carried out until the cleavage. Keep this in mind and move forward.
Good luck!
food for thought nicotine adenine dinucleotide metabolite enzymes possible target for antivirals. pepsin, ribonuclease, trypsin, deoxyribonuclease, pancreatic lipase....
The problem is purifying your target when many impurities are very similar. For really big peptides, like yours, most folks prefer strategies that condense fragments. Sometimes the synthesis yield is even worse with that design, but the final yield after purification is better.
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