Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

How to give noncollinear magnetism terms in INCAR for Double helical magnetic ordering in VASP?

Usually, the magnetic moments of noncollinear tags in the INCAR file look like MAGMOM = 0.0 1.0 0.0 if the atom has 1 unit of the magnetic moment along the b axis. But if we consider the magnetic...

19 November 2020 8,061 3 View

What is physical meaning of Step size in XRD measurement? I am also curious about to how much beam width is kept during X-ray diffraction measurement?

We need to give two different parametrs during XRD pattern measurement i.e. Step size and Step time. Based on this we calculate scan rate of measurement. Is step size and X-ray beam width has any...

25 October 2020 6,157 4 View

What will be the impact on Socio-Economic activities of man after the end of COVID-19 Pandemic?

I am very much thankful of the experts, researcher, and thinkers for very thought full discussion on my previous question on Role of Geography on COVID-19 Pandemic. Now I am keeping another very...

19 October 2020 6,552 4 View

Can someone let me know the best SiRNA along with negative controls for silencing NEAT1 in human breast cancer cells. Company and price if possible?

SiRNA for NEAT1 knockdown in breast cancer cells

05 October 2020 9,723 1 View

I would like some guidance for my project on RECLAMATION OF CONSTRUCTION MATERIALS FROM INDUSTRIAL SLUDGE?

I request the professionals in this to please help me in my project which i am doing in my final year. I'd be very grateful even if its just a bunch of research papers or websites that your...

27 August 2020 3,863 2 View

I am keeping the Question for discussion, What is the role of Geography in the study of COVID-1 pandamic?

Please give your opinion and suggestions

10 August 2020 3,990 19 View

What is the likely impact of AI on future jobs across all sectors?

We have been witnessing the advent of AI and its rapid proliferation as an intelligent machine in many applications from driverless cars, and much more. How is the lifestyle going to change as a...

05 May 2020 4,787 41 View

Commercial materials/surfaces for contact measurement?

Does anyone know of, or use, commercially produced surfaces for contact measurements (control material)? As an example, is there a producer of PTFE discs with highly controlled surface roughness...

09 April 2020 8,806 5 View

What is the inference from the delocalization of P-orbital?

I have attached an image. Can you kindly explain what does it mean from the delocalization of P-orbital from the LUMO plot? I have made a statement " From the LUMO plot it is seen that the...

30 March 2020 9,796 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View