Hi,
What are the difference between these assays and what are the significance of performing the test on microbes cell (fungal pathogens).
Superoxide dismutase (SOD) is an important antioxidative enzyme. It does catalyze the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. Activity of SOD can be determined by a number of methods such as Pulse radiolytic methods (Rigo et al. 1975), Beauchamp and Fridovich (1971); Misra and Fridovich (1972); Tyler (1975). SOD can also be measured by inhibiting the enzyme of an O2- dependent reaction. A number of kits are also now available for measuring SOD.
However, in relation to ROS as well as its relation to microbial cells you can get some knowledge from my recent publications with CAB International, Oxfordshire, UK. There some interesting findings have been illustrated on ROS, antioxidants in relation to microbial cells, specific to AM fungi. I am also forwarding the published paper as it may help you.
Dear Sharifah Farhana Syed Ab Rahman
Pls. find the attached files
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3355102/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713856/
https://www.hindawi.com/journals/jb/2012/217037/
https://en.wikipedia.org/wiki/Superoxide_dismutase
http://www.annualreviews.org/doi/abs/10.1146/annurev.arplant.55.031903.141701?journalCode=arplant
http://www.biotek.com/resources/articles/reactive-oxygen-species.html
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC143417/
http://link.springer.com/article/10.1007/s00294-010-0298-y
Hoping this will be helpful
Regards
Prof. Houda Kawas
Hello,
ROS or reactive oxygen spices s an unwelcome companion of aerobic metabolism. ROS production and its subsequent scavenging - the eqilibrium of these two maintains the homeostasis in any living system. This ROS scavenging machanism has two parts - one among them is enzymatic and SOD or Superoxide dismutase is one among the enzymes.
Now SOD assay will give you a idea about the total concentration of SOD that is been produced by your samples.
ForROS, you can perform DPPH and/or ABTS assay. They will give u idea about antioxidant scavenging ability of your samples.
You will find lots of protocol for these assays online.
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