Hello.
I am studying plant molecular breeding.
I have a question.
Should plant breeders have a ph.D. degree? Only doctors can breed a new cultivars?
I have never heard about plant breeder who is not doctor.
Generally, in my lab, to extract RNA from plant leaf, leople grind the samples with the DEPC treated mortars and liquid nitrogen. However, it takes a long time so I want to skip this stage. Is it...
11 December 2018 7,325 2 View
Hello. In my lab, people use the gel-doc from the Alpha Innotech, and the AlphaImager software is intalled in the public computer. However, the OS of this computer is windows 98 SE so that it is...
10 November 2018 7,461 3 View
In my lab, members usually use large format gel when they perform SDS-PAGE. Some papers reported that low current gave high resolution of the gels, so I tried about 10-20mA with constant current...
02 March 2018 9,501 3 View
Hello. I am studying on plant breeding. In my lab, the others usually are performing researches related to molecular biology and genetics. As far as I know, general process of research is studying...
11 December 2017 5,218 2 View
Hello. I am trying to extract low molecular weight and high molecular weight glutenin subunits. There are many protocols but some of them are similar. Of them, I found two protocols. Their...
07 August 2017 8,648 1 View
When performing the PCR experiments, why don't you use "PCR Master Mixture Calculator"...
06 July 2017 480 1 View
Hi. I am studying the proteins ("A") found commonly in the same tribe. However, there is a exception so that "A" is not found in one genus in the tribe. Instead of "A" protein, the other protein...
04 May 2017 1,788 1 View
Hello. Nowadays, I am studying on some proteins and their genes and their relationship, and their changes with evolution process. Nowadays, I wonder how can I proof the gene disappearing...
04 May 2017 1,808 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...
01 March 2021 8,747 4 View