I am using the nodes for establishing the media for Bamboo sp. I am getting success in bud breaking in MS and BAP liquid media. Further multiplication, I am using the new grown buds in liquid MS/BAP media.But not getting success, please suggest me accordingly. Shall I use again che

Shall I again culture the newly grown node and re-culture again? or put the whole node to new MS solid media?

What mistake I am doing?

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