I am using the nodes for establishing the media for Bamboo sp. I am getting success in bud breaking in MS and BAP liquid media. Further multiplication, I am using the new grown buds in liquid MS/BAP media.But not getting success, please suggest me accordingly. Shall I use again che
Shall I again culture the newly grown node and re-culture again? or put the whole node to new MS solid media?
What mistake I am doing?