Hello, better with FBS. See attached info, may be useful for you.

Hello, better with FBS. See attached info, may be useful for you.

If you are going to evaluate short periods you can use serum with out any problems. However in periods larger than overnight you can have cell proliferation mixed with migration. What I did was to use serum but at low concentrations (1 or 2 %) so that cells survive but don’t divide.

Thank you Dr. Karen and Dr. Diego.