Absolutely RNA Nano kit for small numbers cells ?

The agilent absolutely RNA nano kit, claims to yield RNA from 1-10^4 cells. I have only ever previously used it for 10^4 cells. Has anybody had experience isolating RNA from cell numbers as low as...

30 May 2018 3,164 3 View

What is the working sensitivity range for on-membrane digestion?

13 September 2015 6,456 1 View

What is the validity and reliability of the different sections of the ASSET young offender tool?

Looking for paper on the validity & reliability of sections of the ASSET tool (e.g. substance use) at what it is supposed to measure.

04 May 2015 2,786 2 View

How do I find the concentration of proteins detected after SDS-PAGE?

22 September 2014 5,042 3 View

What is the best way to combine biological replicates from different qPCR plates?

I have been carrying out a qPCR experiment to look for compensatory up-regulation in single deletion mutants of different members of a gene family. Essentially, i want to ask the question 'if gene...

30 May 2013 1,991 12 View

Will you be evaluating microclimates within wilting and wilted flowers?

01 January 1970 7,201 23 View

How can I access SEGAE for my students of sustainable agriculture in Canada?

This seems like a great program. Can it be accessed by students outside of Europe? I'd love to add it as a component of a course that I teach at Trent University in Ontario, Canada.

01 January 1970 5,780 3 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View

What are best free Molecular Dynamics software packages to generate pre-built biological bilayer membranes?

Our task is to numerically invetigate cellular bilayer membrane. In order to numerically simulate behavior of biological complex membrane, we need to have initially constructed membrane. However,...

13 January 2021 7,809 4 View