Design a taqman primers and probes for gene expression assay?

dear all as it is my first time to design a taqman assay , could you recommend for me a good reference to know the good designing for taqman gene expression assay, what is the accepted range for...

15 May 2019 8,287 2 View

Can Chloroform be used as a solvent in cytotoxicity test in cell culture instead of DMSO ?

my drugs can not be dissolved in any other solvent except the Chloroform, so can I use it on cells in MTT cytotoxicity test

09 December 2018 4,922 4 View

How can I estimate the shape and scale parameter of the Generalized Pareto Distribution (GPD) given a specific data-set?

I have already estimated the threshold that I will consider, created the pdf of the extreme values that I will take into account and currently trying to fit to this a Generalized Pareto...

18 May 2017 5,083 3 View

Best way to extract RNA from FFPE human brain tissue?

I am trying to compare yield of fresh vs FFPE and the measure relative quantity by RT-qPCR of housekeeping gene. I can upload the protocol I am using right now if anyone is interested in sharing...

11 December 2016 852 2 View

Should I use isolated RNA or purified mRNA for RT-PCR?

Will isolate RNA from tissue. I will not be running RT-qPCR, but Reverse Transcription PCR. For qPCR typically RNA is used instead of mRNA, I wonder if the same applies to RT-PCR. I only want to...

15 May 2016 3,985 6 View

Any suggestions for Online Learning Libraries in C++?

To be more accurate, I need Online Regression Libraries implemented in C++ (as I want to integrate them into a C++ project). So far I have found sofia-ml and OnlineSVR. Any other suggestions?

08 July 2013 9,397 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View