I was wondering if I might be able to ask the community for some help. I am teaching a class how to use Sequencher to analyze scf files and would like to use some less-than-perfect traces that need a good bit of editing (i.e. a few overlapping sequences with some ambiguous base calls).
I want my students to use the traces to align, smooth out questionable base calls, develop a consensus sequence, and BLAST it to identify the gene.
Does anyone have some they would be willing to share?