S/N role in method development ?

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Ali Samie

Dear Mahboubeh,

Could you please give more detailed explanation about the problem with API? That would be helpful for suggesting a beneficial solution.

Best

Antonina Kokisheva

(S/N) signal to noise ratio is choice to determine LOD and LOQ in HPLC methods.

So, you can proceed using next equations:

LOD= 3 x [C]/(S/N)

LOQ= 10 x [C]/(S/N),

[C] is the concentration of the target compound used to determine the LOD and LOQ and S/N is the signal to noise ratio.