I have extracted RNA from oat, I sent the samples to check the integrity of RNA by bio-analyser. I got more than 28s and 18s bands, people who did the analysis told me that " Then they are quite different from what we know from most other eukaryotes. The automatic RIN algorithm became confused"
what do you think about this, and is it ok for RNAseq or not.
I extract RNA by kit and did another DNase treatment. I attach a picture of one sample.
Thanks alot
Frida Gorreja I got two more bands, with 28s ns 18s. as in the attached file, so my question, can I use these samples for RNAseq
They are extracted from leaf of 2 weeks old plants, with RNA kit from sigma which contain DNAse treatment in its step.
In addition, I made another DNase treatment by Turbo DNase from invitrogene
Frida Gorreja Thanks so much for explanation, I agree that these peaks may be contamination. I will repeat some samples again using different kit and run the gel.
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