Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Which module is better for parallelism numpy functions in Python?

Which you recommend?Numba,Joblib,Dask and etc.Why?

20 March 2020 4,529 4 View

Can anyone advise if there is any company that sells a specific antibody for the HLA DRB1*1501 allele?

Hi everyone, I would appreciate if someone can give me some advise regarding Can anyone advise if there is any company with a specific antibody against HLA DRB1*1501 allele (DR 15 allele HLA...

29 November 2019 1,923 2 View

In Rev manager with continuous data, what number do we have to put in the Mean part while we have two pre and posttest means in the main articles?

I am working with Rev manager 5.3, doing a meta-analysis on 8 studies that each compare 2 psychological treatments and found no difference between them.

25 September 2019 6,804 1 View

Would you remind me some universities or professors for study a phd about food science industry for astronauts?

thank you i am narges heidari.

23 November 2018 3,300 3 View

Have complex values of phonon frequencies any meaning?

I have calculated phonon frequencies of 2D sheet by analytical, classical solution as what we learned from basic solid state texts. But I have complex values for frequencies out of Gama point....

20 April 2018 4,131 6 View

In known group comparison/differences technique, what is the sample size for each group?

I need to use the known group differences to check the validity of the constructs as part of developing a new instrument. The instrument has seven first-order constructs and one second-order...

03 March 2018 4,179 2 View

Does Bio-Resonance really work?

Bioresonance is a non-invasive therapy which involves placing electrodes on your skin for diagnosis and treatment. The electrodes are connected to a Bicom machine which checks the energy...

24 February 2018 6,691 47 View

How can I make CD14 free ( getting rid of CD14 binding) after sorting Monocytes with FACS sorter? Any protocol for max yield and highest viability?

27 September 2016 7,644 2 View

Can we estimate the tail index of stable distribution with an estimator which is based on GEV distribution??

18 February 2015 7,438 3 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View