I am running an enzyme assay in Perkin Elmer Victor 2D fluorometer. The device displays its results in units called "Counts". I have run a set of methylumbelliferone standards and recorded the Counts. Some research papers that I've used for reference have plotted standard graphs using RFU on the Y axis. Now do I have to convert the Counts into RFU or can I plot my Standard graph using Fluorescence count itself?
If conversion is needed, how do I calculate RFU from Fluorescence counts?
Hi Harshitha S S ,
Are you sure that what you got were fluorescence count?
Because, it may be fluorescence intensity (FI).
And if it is FI then you can use it.
Thank you,
Good luck
The "count" could be from events counted by the photomultiplier tube (PMT).
However, the unit of RFU is arbitrary and simply indicates the "amount" of light (of a particular wavelenth) detected by the instrument. So I don't see any problem. You can call it "counts" (assuming that these are in fact PMT events, or even events recalibrated to mean actual photons) or RFU (what hides the useful aspect that these are PMT events or photon counts - if this is the case).
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