How to join three domains of a protein together?

I have modelled three domains of a protein separately, now I need to join them together for the final protein. I was thinking of loop modelling of the linker protein. Can anyone suggest me some...

06 December 2019 121 2 View

What could be the reason for observing a band in NTC in RTPCR?

We have been getting Ct values almost similar to a test sample in NTC wells. The melt curve also overlaps with the test samples. While running a gel, a band with a product size similar to the...

02 March 2017 5,144 3 View

Can I reuse the calcein labelled tissue within PMMA blocks for decalcification and perform routine histology?

I will be tracking the bone velocity in vivo using Calcein injection in animals. I understand that the sections should be fixed in 70% ethanol before embedding them in PMMA to get the sections....

14 March 2016 1,609 8 View

Is cloning a 9kb 3'UTR for luciferase assay necessary or cloning the regions of 3'UTR-miRNA interaction is sufficient?

08 February 2016 3,164 3 View

Can anyone suggest a protocol for RNA isolation from bones?

Usually I homogenize the bone fragments and proceed with conventional RNA isolation using TRI. But I find the purity of the RNA to be low. Which is the ideal method for storing bone samples and...

01 March 2015 4,870 7 View

How should I go about designing a PI controller necessary for the PWM of an inverter operation?

This is the PI controller that is used in the Park's transformation.

09 December 2014 3,773 2 View

What is the best way to transport RNA samples abroad?

17 October 2014 8,364 5 View

To what extent would the number of passage alter the miRNA expression?

There are many studies reporting the alteration in miRNA expression in the long-term cultures. But will there be any significant changes even when P2/P3 MSC cells are used for differentiation...

30 April 2014 7,483 1 View

Is there a possibility to screen the signaling molecules within bone marrow filtrate?

How to study the presence of a particular pathway in bone marrow filtrate?

30 March 2014 8,709 1 View

Intralipid infusion for recurrent pregnancy loss?

what is the phsiological role of soyabean and NK cells

09 January 2012 4,581 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

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02 March 2021 324 2 View

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Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View