Refractive index, viscosity and dielectric constant of nutrient broth media ?

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Alan F Rawle

Drishti Garg

The RI of the particles is not required for the size intensity distribution in DLS. If it’s the medium that you’re suspending the particles in then the RI will be close to water (1.33) and can be measured with an Abbe refractometer.

@alan rawle thanks for your reply. Yes, RI won't effect much and will be closer to water. I don't have Abbe refractometer. Secondly, what will be the viscosity and dielectric constant for nutrient broth and nematode growth media.

Again you can measure the viscosity with a viscometer or rheometer. Or see Article Simultaneous Measurement of Viscosity and Optical Density of...

Ulf Nobbmann

Hi Drishti Garg , the first approximation would be to take the values of water. The next improved approximation would be to use the "complex Solvent Builder" in the Zetasizer Nano software and predict the viscosity etc. based on the (major) components in your nutrient broth. And finally, you could potentially measure the viscosity indirectly with probe beads of known size. See for example Article Measuring sub nanometre sizes using dynamic light scattering

and the complex solvent builder at https://www.materials-talks.com/blog/2011/08/04/getting-ready-for-a-zetasizer-demo/

Ulf Nobbmann Thanks for your reply sir. Yes, I checked that too. However, I could not get through because the composition of nutrient broth is - peptone (5 grams/litre) , sodium chloride (5 grams/litre), yeast extract (1.5 grams/litre), and beef extract (1.5 grams/ litre). Out of which, none of the additives were found in the software barring NaCl. How may I proceed now sir?

Regards

Drishti

Your composition contains only a total of 13g/L of material. This is 1.3%. Use (and report) the properties of water. Yeast and beef extracts are not necessarily pure materials anyways and not available in AR grade...

Alan F Rawle Ulf Nobbmann Thank you sir.