Are recombinant monoclonal antibodies utilized for the capture and detection antibodies in R&D Systems DuoSet ELISA kits (sandwich format), or is there a preference for both polyclonal and monoclonal antibodies?
Hi All, Can anyone recommend a Delphi method online tool that I can use? Which one is more user-friendly and cheaper? I am also wondering, is there any way to make an online Delphi survey for...
01 April 2024 4,673 0 View
I will use molecular dynamics simulation for the refinement of more than two protein structures. Most of the research for refinement in the literature is about the docking of two proteins. So I...
23 March 2021 6,109 3 View
Hi all, I am doing some preliminary calculations on quantum espresso about TMN4 structures on defective graphene layer (26 carbon, 4 nitrogen, 1 transition metal atom). While geometric features...
10 May 2020 7,043 0 View
If you are using CTD-Diver, can you tell me which brand and what are the advantages and disadvantages of your equipment?
07 July 2019 9,828 0 View
Does anyone know the survival rate and duration of Mytilus galloprovincialis between 30 and 40 m depths?
22 March 2018 6,728 4 View
Lifestyle and cognition are very much conected ,what would be the most up to date questionnaire taking in consideration all the factors involved
23 February 2018 5,291 3 View
Epidemiology population study methods of inquiry the proper number to be statistically important etc
28 October 2017 7,196 8 View
Starting anticoagulants after FA related acute ishemik stroke with transforming hemorrhage
03 September 2017 3,307 4 View
Read 6 answers by scientists to the question asked by Dildar Bahar Genc on Nov 24, 2015
23 November 2015 8,064 6 View
I need to categorise the information on CSR reports and I am following several models which recommend different types of categorisation. What are the fields that a CSR report should contain and...
24 September 2015 8,803 5 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
through genetic modification or metabolic engineering? Is it helpful, good for health ?
22 July 2024 7,811 4 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View