Hello to everybody,
the so-called reactive dyes were designed to bind covalently the cellulose of clothes in certain conditions (in general basic pH and heat). I'm wondering if it's realistic to imagine that they can also react in a similar way with the agarose of the medium during for example an Ames test. Obviousy the reactivity is strictly dependent to the specific structure but is it lecit to assume that this reaction can probably take place also in milder conditions?
Attached there are two examples of chlorotriazine- and sulfone ethyl sulfate-based reactive dyes.
If someone would like to share his/her personal opinion it would help!
Nicola
Hi Nicola. In general OH-polymers are not very reactive and I doubt that the reaction mechanisms that you gave will work well with OH polymers.
I recommend the book "Bioconjugate Techniques" by Greg T. Hermanson for further reading.
Really thanks for your kind reply and the advice of reading.
The reaction actually works on cellulose and basic reaction environment. Chlorotriazine- based dyes are quite old compounds since they were developed during the Fifties but they are used also nowadays and the reaction is known.
My reasoning is due to the similarity between celllulose and agarose.
Sincerely,
Nicola Porta
Hi Nicola,
It is possible to dye agarose with triazine dyes as well as using vinyl sulfone dyes. The reactive group is the primary OH in agarose. I have in the past dyed sodium alignate which is similar to agarose using Cibacron F dyes from Huntsman Corporation. Prepare 0.2% dye solution (pH 11 with mild alkali preferably sodium carbonate to reduce hydrolysis of the dye) in the presence of approx. 2%NaCl. Treat the agarose gel with this solution at room temperature for 2-3 days. if you use Procion dyes, then only 2hrs will be enough as the are highly reactive.
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