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Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...
31 May 2024 1,088 0 View
Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...
26 May 2024 4,753 2 View
I attempted a phosphate (apatite) flotation, but the slurry was too thick for the impeller to work properly. I reduced the solid content to 30% for better mixing, but the conditioning still seems...
14 May 2024 4,505 4 View
Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...
15 April 2024 6,778 3 View
Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...
11 March 2024 9,171 8 View
Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...
10 March 2024 8,764 0 View
Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...
19 February 2024 1,672 5 View
Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...
19 February 2024 9,305 2 View
Hello All, I have shRNA lentivirus particles and I want to produce self-inactivating replication incompetent viral particles in cell by myself. My purpose is to produce knock-down cells in flask...
10 January 2024 6,425 0 View
eliminoar articulos que son de tu autoria
05 July 2024 2,107 1 View
Economic factors influence family dynamics.
02 July 2024 6,235 0 View
karma
20 June 2024 6,189 1 View
Es posible observar que mientras que paises como colombia con altos indices de pobreza y desigualdad, abrieron sus fronteras a millones de migrantes venezolanos, por otro lado algunos paises de la...
16 June 2024 236 2 View
Hello, I duplicated my research account by mistake recently. The recent one shows 2 research publications. I would like to delete it immediately but I don't know how. Can you assist please? I...
28 May 2024 905 0 View
Estoy realizando un curso de Divulgación y Marca Personal del G9 en el que me solicitan hacer una pregunta abierta en ResearchGate.
19 May 2024 4,290 1 View
En mi opinión son respeto y responsabilidad ambiental y usted que opina. Le adjunto dos publicaciones de mi autoría, para que me pueda dar su criterio al respecto.
07 May 2024 6,006 1 View
I have trtriedy some sites like World Development Indicators, IMF, African Developmennt Banks/ Group. Data needed are: Interest rate (Nominal or other wise), financial development data, Finanacial...
04 May 2024 373 7 View
Je dois rédiger et soumettre mon mémoire de Master sur le thème Gouvernance et Gestion et gestion socio-environnementale et économique. Mon sujet porte sur la gestion de la brèche de la langue de...
02 May 2024 4,815 1 View
antecedentes de investigaciones relacionadas con la pregunta y el restreo bibliografico que soporte esta pregunta.
01 May 2024 4,237 0 View
