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When purifying total RNA from yeast that have grown on nitrogen-limited medium, it always appears more degraded when coming from stationary phase than from log phase. RNA coming from log phase...
09 April 2023 3,899 2 View
In the latest edition (2016) of the Basic Practical Microbiology Manual from the Microbiology Society, all the aseptic procedures are carried on near a Bunsen burner. This is the common practice...
27 March 2023 4,863 6 View
I've read that I need cDNA standard curves to test primer efficencies. I'd like to know if I could use genomic DNA instead, since I've got too many different conditions from which different cDNAs...
27 November 2022 5,289 4 View
From what I see in most of the commercial houses the WB usually give up to 3-4 bands ranging from 21,34,40,70 kDa. I need it for WB and IF in Tissues samples. Based on your experience, would...
30 August 2022 1,248 0 View
Which NGS technology (if viable) would be most useful in confirming presence/absence of virus contamination in cell banks used for drug production? I imagine it would include WGS of host cell...
05 July 2022 9,016 0 View
I have one PCR workstation. I performed my RNA extraction/cDNA synthesis on a separate bench (outside hood). I now have very concentrated cDNA. In previous labs I had 3 work stations. 1: Prepare...
05 May 2022 3,235 1 View
The reason for my email is because I am a PhD student and I study emotional intelligence in alcohol and drug addicts, I want to know if you have the emotional quotient inventory of 133 questions...
01 March 2020 3,830 3 View
Am using a 160mM HEPES buffer and diluting serum by 10x and 200x. Found this [ ] works with blood. pH of buffer is 7.4 and serum was kept at either 4C or RT. Disperant T was altered from 4C to...
18 November 2019 8,484 3 View
I have been reading about the observer effect as conceptualised in Physics. However, I would like to know how this concept has been applied in the social sciences.
05 June 2019 7,418 5 View
04 June 2019 2,595 4 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View
I am currently researching determinants of China OFDI; and host country determinants using sectoral-level data.
23 July 2024 8,729 3 View
In memory of a worthy Chinese scholar
06 June 2024 2,098 0 View
The IMF allocates SDR quotas to supplement the reserves of its member countries. IMF added Chinese renminbi, as an international reserve currency beginning in October 2016 (which many believe was...
02 June 2024 4,948 0 View
Dear colleagues I have to prepare a document for Oxford University Publishing group that follows an unusual way of listing references. It requires that references meet two conditions: All are...
28 May 2024 5,612 4 View
Could anyone help me in identification of these species growing in China's coastal wetlands? I attach some photos. Thank you.
27 May 2024 9,656 7 View
Are the licensing rounds that China recently obtained from Iraq considered a victory for the Chinese pole over the American pole?
14 May 2024 3,151 2 View
"Artistic Dreams and Paintings - Colorful Seeds" children's art exhibition will be held in Hall 1, 2 and 3 of Hunan Art Museum, China, from July 12 to August 20, 2024. This is the first exhibition...
05 May 2024 4,314 0 View
There was a database from a university in China, but it seems no longer accessible to the public. Are there any other database to search for bivalent genes in human? Thanks.
24 April 2024 976 0 View
Background: A Chinese company (DT) is seeking market development opportunities in Southeast Asia, the Middle East, and Eastern Europe. Before conducting business, it requires communication...
24 April 2024 6,815 0 View