Since I'm a newbie to qPCR I wonder if anyone could help me with result interpretation. I have a relative standard curve experiment where expression of gene of interest (pcsA) is tested within a range of changing conditions (choline concentration in medium). Till now I had always obtained results where difference in expression level was significant (20 - 100 fold change). Here difference is, lets say, moderate (max 2x). I wonder if this can be interpreted as any change or one should rather claim that expression is at the same level. The experiment was done in triplicate. I attach the graph. thank you in advance for your precious help!.