I am in qiime1, trying to get some sequence pre processing done with my 16S data. I have both the forward (R1) and (R2) sequences running with the following :
multiple_join_paired_ends.py
using this website as a guide
http://www.metagenomics.wiki/tools/16s/qiime/pre-processing
i entered the following into qiime1:
multiple_join_paired_ends.py -i u -o p
and got this error:
(qiime1) codea@lsp-bioinfo03:~/r_drive$ multiple_join_paired_ends.py -i u -o pTraceback (most recent call last): File "/home/codea/miniconda2/envs/qiime1/bin/multiple_join_paired_ends.py", line 4, in __import__('pkg_resources').run_script('qiime==1.9.1', 'multiple_join_paired_ends.py') File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/pkg_resources/__init__.py", line 657, in run_script self.require(requires)[0].run_script(script_name, ns) File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/pkg_resources/__init__.py", line 1437, in run_script exec(code, namespace, namespace) File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime-1.9.1-py2.7.egg-info/scripts/multiple_join_paired_ends.py", line 201, in main() File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime-1.9.1-py2.7.egg-info/scripts/multiple_join_paired_ends.py", line 193, in main qiime_config=qiime_config) File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/util.py", line 46, in __init__ self.write('QIIME version: %s\n\n' % get_qiime_library_version()) File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/util.py", line 57, in write self._f.flush() IOError: [Errno 95] Operation not supported
(qiime1) codea@lsp-bioinfo03:~/r_drive$
can anyone help me find out what this error is? I have reinstalled qiime today and still get the same result. I am following the instructions of the website as mentioned above, and have undergone all the file name conversions it asks for.
any help is more than appreciated.
Thanks.
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