I am running qiime1 on my university server. I am wanting to do some raw data pre processing with link instructions here http://www.metagenomics.wiki/tools/16s/qiime/pre-processing.
I have followed the links instructions to this point:
- # merge forward and reverse reads (multiple samples)
multiple_join_paired_ends.py -i IlluminaPairedReads -o JoinedReads
but i am met with this error:
(qiime1) codea@lsp-bioinfo03:~$ cd r_drive
(qiime1) codea@lsp-bioinfo03:~/r_drive$ multiple_join_paired_ends.py -i IlluminaPairedReads -o JoinedReads
Traceback (most recent call last):
File "/home/codea/miniconda2/envs/qiime1/bin/multiple_join_paired_ends.py", line 4, in
__import__('pkg_resources').run_script('qiime==1.9.1', 'multiple_join_paired_ends.py')
File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/pkg_resources/__init__.py", line 657, in run_script
self.require(requires)[0].run_script(script_name, ns)
File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/pkg_resources/__init__.py", line 1437, in run_script
exec(code, namespace, namespace)
File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime-1.9.1-py2.7.egg-info/scripts/multiple_join_paired_ends.py", line 201, in
main()
File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime-1.9.1-py2.7.egg-info/scripts/multiple_join_paired_ends.py", line 193, in main
qiime_config=qiime_config)
File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/util.py", line 46, in __init__
self.write('QIIME version: %s\n\n' % get_qiime_library_version())
File "/home/codea/miniconda2/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/util.py", line 57, in write
self._f.flush()
IOError: [Errno 95] Operation not supported
(qiime1) codea@lsp-bioinfo03:~/r_drive$
can anyone help me find out what im doing wrong?
( i have limited python/qiime experience)
screenshot also attached
Any help appreciated.
The reason it wasn't working was i was running everything from a shared server folder. This wont work, as windows will conflict with Linux. This has since fixed the issue
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