We would like to order an E. Coli strain with a specific serotype (O113 H10 K negative), but I had not success when I search for this in repositories like ATCC and others. This particular E Coli serotype is used frequently to extract LPS. Any suggestion where I might be able to buy this is highly appreciated.
So I do not want to buy LPS/endotoxin, I want to buy an E Coli strain with a particular serotype I can culture...
You might take a look through the available strains at The Coli Genetic Stock Center (http://cgsc2.biology.yale.edu). They do not charge much, mostly handling/shipping fees.
Well, it seems like this particular strain is not freely available. See here, for example (https://govtribe.com/project/cgmp-endotoxin-for-clinical-trials):
"... List Biological Laboratories, Inc. is the only known vendor/service provider that can manufacture high quality, lipopolysaccharide (LPS) isolated from E. coli O113:H10: K- ... E.coli 0113 O113:H10: K- strain will be provided by the vendor".
SSI Diagnostica may sell it (http://www.ssidiagnostica.com/e-coli-strains/?cid=44):
"... We primarily sell reference strains and have reference strains for all O, H, K and F antigens, various toxins and other E. coli virulence factors.
The strain collection also contains wild type strains representing a wide selection of the possible serotypes and virulence factors in various combinations. When ordering a wild type strain, it is important to specify which antigen/virulence combinations are required, as our strain bank, as mentioned, consists of more than 64,000 strains".
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