What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why would one go up and then down with gradient during HPLC?

I think I've seen it previously, but the current example is from this publication https://www.mdpi.com/1420-3049/14/5/1825 where they used the following gradient on HPLC: min ... % MeOH 0 ... 2 20...

09 February 2021 5,422 3 View

How can you alter line thickness in emmip plot (R)?

I am working with R and plotting emmeans graphs, yet I cannot find online how we can change the line type or its attributes using the emmip function. I have two independent categorical variables,...

27 October 2020 9,339 1 View

How to synchronize two stepper motors most optimally?

We need to push a large load (2 tones) using two stepper motors, connected rigidly to the axis (two screws). I'm worried the motors could micro-step out of sync. And cause malfunction. I can see...

21 October 2020 6,363 4 View

How to fix convergence problmes using Mohr Coulomb Plasticity?

Dear colleagues, I am trying to simulate a strip foundation on a cohesionless soil with a friction angle of 30 degrees. However, I obtain convergence problems. I am using a geostatic step. The...

21 October 2020 4,556 2 View

Can you integrate Zotero with this website?

Is it possible to integrate Zotero or any other bibliographic software application with this website application? If not, is it possible to do that with academia, ssrn or Google Scholar? Any...

21 October 2020 4,712 1 View

How to find out, if a recent Sentinel-2 acquisition failed or had a problem?

Hello, I am currently waiting for an image to appear on Scihub and it takes longer than usual (acquisition date 2020-09-22, orbit r122, T33UWR tile actually, but looking for general answer). So it...

23 September 2020 6,842 5 View

Can small law firms adopt AI?

After considerable research, I have found several use cases which can be used to exemplify some implementations of AI on law firms. However, there is clearly a disadvantage for this segment when...

01 September 2020 7,132 5 View

How can i model a shock tube with 2 different gases on Fluent?

Hi, I'm currently trying to study shock tube analysis on fluent and I have modelled a shock tube with a driver section (high-pressure) and a driven section (low pressure). What I want to study is...

22 July 2020 8,162 3 View

How can i make an isopeptide bond in GROMACS ?

Hi everyone I'm new in all the field of molecular dynamics, but i trying to run a SMD of one protein with two isopeptide bond, one in each domain, that prevent my protein stretch. My problem is...

07 July 2020 6,201 3 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View

Could someone please provide me with a reliable differentiation protocol for IMR-32 NB cells?

Treatment with 10uM Retinoic acid seems to result in vacuolization and cell death

20 January 2021 4,013 2 View

How to I carry out tobit regression on STATA with Simpson index as my dependent variable?

Hello everyone, I am working on the topic; Impact of Income Diversification on the Livelihood of Small Holder Dairy Farmers. One of my objectives is to evaluate the determinants of income...

04 January 2021 1,388 2 View

Does early COVID 19 treatment matter ?

The SARS-CoV-2 virus spreading across the world has led to surges of COVID-19 illness, hospitalizations, and death. The complex and multifaceted pathophysiology of life-threatening COVID-19...

29 December 2020 7,446 4 View

What is your understanding about death ?

Do you think of it as a part of life, as a disease without a cure, as a way of immortalization, as a way of transformation, as a way to freedom..or something else. Is it good or bad? We all know...

22 December 2020 2,041 37 View

What can be used as a positive control for Annexin V/ 7AAD stain with BL6 mice STAT3 Knock Out B-cells?

I am trying to isolate B-cells from the spleen of a STAT3 KO mouse and plan to stain with Annexin V and 7-AAD. I will then use flow to determine apoptosis and overall cell death. I have used...

18 December 2020 9,600 3 View

Best method to detect cell death in the brain of adult zebrafish on sections?

Hello all, I am trying to detect cell death in the brain of adult zebrafish on sections. I performed immunohistochemistry on brain sections using the Purified Rabbit Anti- Active Caspase-3...

14 December 2020 9,940 3 View

In SPSS, how to select cases if variable has x but not y?

Hi all, I have an SPSS longitudinal database in which one of the variables is monthnumber. The month ranges from 0 (baseline) through all of the months they were involved and then 51 for last...

30 November 2020 490 4 View

Why do my ARPE-19 Cells die after GFP-Plasmid (pEGFP-N1-FLAG) transfection?

Good day everybody, i am currently trying to transfect ARPE-19 cells with a GFP Plasmid (pEGFP-N1-FLAG; Plasmid #60360). I am using Viafect which works really well (transfection rate of over 95...

29 November 2020 2,976 6 View