Does anyone can share the best protocol or procedure for measuring Terpenoids and total alkaloid? or any website for suggestion? Any help is highly appreciated and I would like to thanks in advance.
For alkaloidsthe material is extracted with alcohol, solvent distilled off under reduced pressure and the residue treated with dilute inorganic acid, whereupon the bases are extracted as their soluble salts. The free baes are liberated by the addition of sodium carbonateand extracted with various solvents like chloroform.
Terpene are volatile low boiling compounds. The material is subjected to steam distillation and the distillate(oil) is separated from the aqueous phase.