Protocol for polymer stainning with Ruthenium tetroxiode?

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Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

How do I determine the mg of ascorbic acid equivalent to 1.0 mL of standard indophenol solution?

Hello everyone. Could you help me with the following problem. I am going to determine ascorbic acid in capsules using an AOAC method. The method gives the following equation: mg ascorbic...

24 June 2024 789 1 View

What are the best ways to the recount of microalgae?

I know the neubauer method, but I hear about the recount of growth with spectrophotometer.

23 June 2024 9,295 1 View

Metodology for dashboard, please?

Please scientific articles on methodologies to create dashboards in R shiny and PowerBI

27 May 2024 387 1 View

No correlation between the independent variables and the dependent variable?

I want to conduct a multivariable linear regression analysis with 15 independent variables, however when I was checking the assumptions not a single IV correlates with the dependent variable. Can...

14 May 2024 5,151 7 View

What are the UV ranges and temperatures mostly effect on microplastic degradation?

For a study about the effect of UV and temperature on plastic degradation and to compare the changes between different UV and temperature levels by using fresh plastic pellets. What would be the...

22 March 2024 4,067 1 View

¿Cómo se implementaría una metodología participativa que involucre a personas mayores en el diseño y desarrollo de programas de bienestar Social?

Nos enfocamos en como diseñar estrategias participativas para involucrar a los mayores, y lo más importante es que respete su autonomía y dignidad. Buscamos entender como incluirlos en la toma de...

10 March 2024 2,659 3 View

Do you have seed trait data for tropical species?

We would like to invite you to build an inclusive, open-access database on seed traits for tropical species. If you have morphological, biophysical, or germination data for tropical species,...

07 March 2024 2,897 4 View

Você trabalha com traços funcionais de plantas de Cerrado?

Nós gostaríamos de te convidar para participar e contribuir com com o Cerrado Plant Traits: uma rede para a construção de um banco de dados de traços funcionais de plantas. Se você tem dados...

27 February 2024 2,142 2 View

DDS and Pharmaceutical industry. Conferences opinions?

Hello everyone! I am considering the possibility of assisting Congress or Conferences on Drug Delivery Systems or Pharmaceutical Formulation. However, I do not have much experience in this...

30 January 2024 7,440 0 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Polymer wear calculation?

What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?

04 August 2024 1,078 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View