I cannot find a comprehensive protocol for stainning polymers with Ruthenium tetroxide solution. I would like to know the instructions and safety precautions for stainning and handling of Ruthenium tetroxide solution.
You might want to read this.
https://www.2spi.com/item/02592-ab/
Here is a paper using RuO4 to stain polymers.
https://core.ac.uk/download/pdf/11452858.pdf
you might want to request this from ResearchGate:
Chapter Transmission Electron Microscope Studies of Polymers Stained...
I want to process a very large file, let's say 300 GB, with Python and I'm wondering what is the best way to do it. One limitation is that my algorithm needs the whole file in memory, so a...
15 January 2021 6,691 4 View
Hello everyone, I am currently working on the natural colour pigment, betalains. I am looking for 96- well plate adopted assay protocols for measuring superoxide and hydroxyl radical scavenging...
03 January 2021 2,414 2 View
Usually industrial policy is interpreted as changing economic structure from in favor of more productive/advanced economic activities. But would like to read something that discusses the...
31 December 2020 3,199 3 View
I would like to simulate the Lorentz Force resulting from the magnetohydrodynamics (MHD) phenomenon in an ionic solution.
19 December 2020 8,278 9 View
Greetings! I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA...
02 December 2020 1,310 2 View
Good Morning! Has anyone compared supervised classification methods using Google Earth Engine? Could you comment on what are the criteria you used to decide on any method?
29 November 2020 544 4 View
Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...
18 November 2020 3,867 3 View
I am trying to publish a couple of articles in order to improve my scientist CV...
23 August 2020 4,264 16 View
Hello. I'd like to perform a Western Blont with samples obtained from 3D culture in matrigel. I've done experiments with 3D cultures in 96 well plates in order to test drug activity, but now I'm...
27 July 2020 1,421 4 View
I am trying to simulate the impact of a 9mm bullet into a composite metal foam (steel) panel. I might ask more questions in the near future so if anyone with knowledge about the topic and free...
24 July 2020 6,478 2 View
I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...
03 March 2021 7,540 4 View
Anyone can recommend any books on such topics? Thanks in advance.
02 March 2021 8,457 2 View
Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...
02 March 2021 6,186 3 View
I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...
02 March 2021 1,404 6 View
Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...
28 February 2021 7,882 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...
28 February 2021 298 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View