Smart VacPrep, anyone had problems with degassing?

12 October 2020 1,907 2 View

Anyone know an effecient method for transfection / siRNA knockdown in organ culture (retina)?

Hello everyone, I have made a nice protocol for culturing whole adult rat retinas in vitro. This is also called organ cultures or whole mount cultures. The retinas are sitting on a filter in a...

15 July 2020 5,300 1 View

Factors affecting precipitation of serum proteins?

Hello, In most modern protocols for metabolomics/lipidomics proteins are precipitated using solvents such as methanol, acetonitrile or isopropanol. After vortexing/mixing the next step is often...

28 April 2020 3,383 2 View

Personality. Enherited or socially constructed?

What is your take?

28 July 2019 7,443 17 View

DNA barcoding: is there a COI alignment that spans the Eukaryota?

Regarding the DNA barcode COI (cytochrome oxidase subunit 1), I'm looking for a multiple sequence alignment that, ideally, spans the Eukaryota. Is there any such alignment available for...

30 September 2017 506 5 View

How can you get rid of air bubbles (or blisters) on butyl compound used for inner tubes?

Looking for a method to eliminate air bubbles on butyl inner tube? there are blisters on cured butyl inner tubes, is this a compounding problem or machinery process?

16 September 2016 5,717 2 View

Should I use "locus", "gene", or "marker"?

20 March 2014 8,234 4 View

International business, further globalisation or backlash?

In the field of international business the trend has been a move towards globalisation and integration. However research has shown that lately most government policies have been of a protectionist...

01 January 1970 8,882 59 View

Ambivalence: Where to draw the line between the pathological and the non pathological

When it comes to Ambivalence, it can be a symptom of mental illness but it is also something we experience in our everyday life ( I certainly do, and my wife hates it) . Where would you draw the...

01 January 1970 6,670 9 View

Should we ban research conducted on animals :Animal testing?

As a vegetarian but also as a human Im ashamed when I see pictures of animals used in cruel experiments. I find it utterly disturbing and in all honesty sickening. What are your thoughts? How do...

01 January 1970 2,533 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View