Is it correct to asume that a protein-lipid complex moves slower than a protein alone in FRAP experiments?, briefly, I have a membrane channel fused with YFP, the channel has the capacity to bind diacylglycerol (DAG). Would you consider that under a DAG production situation, a WT channel will move slower (or lower halftime recovery) than a mutant channel that can't bind DAG? Thanks a lot in advance for your answers and suggestions.
Javier