PRINCIPLE of ether-alcohol mixture when homogenizing brain tissue. Why alcoholic KOH added to ether-alcohol extract?

11 September 2020 0 4K Report

EXTRACTION OF THE TOTAL LIPIDS: Explain the principle behind using ether-alcohol mixture when homogenizing a pig's brain in this experiment.

ISOLATION OF TRIGLYCERIDES: Why is alcoholic KOH added to the ether-alcohol extract? What is the chemical reaction?

This mixture will be heated for a long time, until saponification is complete. When 1-2 drops of saponified solution is placed in 3 ml of water, what is expected to happen?

The saponified solution is heated again until the alcohol is driven off. The resulting syrupy material will be used for the next steps.

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Rodolfo Ii Carreon's questions See All

How can I find out how to reach a person by email to send them full texts of my articles?

How can I find out how to reach a person who wants full texts of one of my several articles listed on ResearchGate, so I may email the requestors one of my several articles they are requesting?

27 February 2024 1,957 0 View

Δ15N fractioning records have negatives values, what that means it?

Hello I have 94 sediment (2cm slices) from cores obtained in four mangroves drainages of northern ecuadorean coast, that exhibit atypical values of δ15N (negative values) as well as higher...

27 January 2024 8,710 0 View

How can I do a correlational analysis on two different Likert scales?

I use Accessibility Usabaility Scale on Level of Accessibility and Usability for each communication channels (television, radio, social media, government websites, and print media) in obtaining...

18 November 2023 4,477 3 View

Information of Feeding of ecuadorean freshwater fishes?

Hello, maybe somebody could provide me information about the feeding of this freshwater fishes of the ecuadorean coast? Bryconamericus dahli Brycon dentex Brycon sp Chaestostoma Mesoheros...

16 November 2023 2,828 2 View

What is brain learning?

theories on brain learning

09 November 2023 7,474 0 View

References on how to comment a quotation in academic paper?

I need references on how to comment a quotation in academic paper and kinds of comments.

26 October 2023 5,364 1 View

How to evaluate submerged aquatic vegetation vs factors using braun-blanquet like coverage data?

Let's say I have SAV (~4spp) cover on a Braun-blanquet like scale for several years & I want to evaluate the effect of sediment characteristics, would a logistic model be the right approach? I...

16 July 2023 783 0 View

Could someone recommend me an easy-to-prepare solution to preserve gelatinous holoplankton?

Ctenophores and other gelatinous holoplankton beings obtained with planktonic meshes burst when adding 70% alcohol

15 July 2023 9,894 0 View

How to eliminate a contamination faint band on negative control?

We performed several PCR assays to detect virulence factor of pathogenic strains, but a faint band amplified on negative control. After this, we have changed all reaction components (one by one)...

26 April 2023 6,640 3 View

What is the best sonication method to obtain highly concentrated graphene oxide water dispersions?

I would like to ask about the best conditions of sonication of graphite oxide in terms of apparatus (probe or bath), time and power in order to effectively exfoliate the graphite oxide without...

29 November 2022 2,767 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View