I'm looking for well studies primers and probes for qPRC diagnosis of:
Blumeria graminis,
Puccinia recondita,
Septoria tritici,
Septoria nodorum,
Rhynchosporium secalis,
Pyrenophora teres,
Puccinia coronata,
Sclerotinia sclerotiorum,
Alternaria brassicae,
Phoma lingam,
Botrytis cinerea,
Plasmodiophora brassicae,
Helminthosporium turcicum,
Aureobasidium zeae,
Cercospora beticola, and
Phytophthora infestans
Hi,
which kind of primers are you searching for?
Are you asking for a type of primer that works for all the listed pathogens or one that work for each of them?
Which kind of qPCR are you working with, TaqMan or Sybr Green based ?
Best regards
Hi.
I'm looking for one pair of primers for each pathogen. TaqMan assays with probes will be perfect, but I know that it can be impossible, so Sybr Green is also to do.
Hi,
have you tried already on NCBI search? You might find the probes for the specific pathogen.
Follow the link:
https://www.ncbi.nlm.nih.gov/probe?
https://www.ncbi.nlm.nih.gov/probe/?term=Blumeria+graminis
- Badges
- Science topic
- Similar topics
- Philosophy
- Philosophy Of Science
- Reasoning