Primary amine detection in silk fibroin?

More Mina Ghorbani's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Methods of O-methylation of amino acids without interfering the amine groups?

Hello guys. I have faced the problem of being in need of carboxyl-methylated amino acids. Amino acids I need are not available in the market so I need to synthesize them by myself. So thus I'm...

16 June 2024 9,920 3 View

How to purify Schiff base product?

How to stabilise the Schiff base product and avoid it from reverse back to the aldehyde and primary amine.

09 June 2024 9,264 5 View

How to select aminolysis reaction over transamidation reaction bw an amine and an amide ester?

Hi. i have a molecule it has both amide and ester bond. When i want to get second amide bond with ester part of the molecule by an amine, but it attacks to the amide bond also. In which conditions...

01 June 2024 9,863 2 View

Does anyone know how to Coat polystyrene particles with APTES ?

I want to have amine group attached on the polystyrene particle surface. APTES can be used to do that.

21 May 2024 8,092 0 View

Please suggest procedure for deprotection by HCL in dioxane to make amine ?

Free amine

26 April 2024 4,950 0 View

How can you avoid triethylamine salt precipitation while protecting Secondary amine with acid halide in presence of NEt3 especially in Flow sytem?

protecting Secondary amine with acid halide in presence of NEt3 especially in Flow sytem?

23 April 2024 5,746 1 View

Which solvents and what ratio should I use for TLC to seperate my azo schiff base and azo aldehyde?

I have synthesized an azo Schiff base using an azo aldehyde and an amine. Now I can not separate my compound in TLC plate. I have used n-hexane and ethyl acetate mixture, acetone, and n-hexane...

24 March 2024 1,099 3 View

What could be the possible reason for the biexponential fluorescence lifetime decay, observed in nonpolar solvents in case of few amine derivatives?

In non-polar solvents such as cyclohexane or heptane, we are observing biexponential fluorescence lifetime decay in few amine derivatives, which is an unusual observation. Could someone be able to...

17 March 2024 8,288 3 View

Kumar Chokalingam

You can use a ninhydrin assay

Mina Ghorbani

Thanks

Based on my researches, I found that the ninhydrin assay detects both primary and secondary amine.

If I'm right, I can't conclude the presence of primary amine from this test, while there is also secondary amine in silk fibroin structure.

Abdelkader BOUAZIZ

Dear all, the following chapter resumes the different techniques for amines detection. My Regards

I really appreciate your help.

Thank you