What can I do for the re-isolation of phages and separation of mixed DNA of bacteriophages?

Dear researchers During my thesis isolation of phage and also re-isolation from one plaque and passaging of that to reach a high concentration, I extracted the DNA and catch TEM. My TEM images...

09 June 2020 1,747 6 View

How to conductive polyethersulfone membrane?

Please suggest a way to conduct low voltage poly ether sulfon membrane.

24 December 2019 5,108 3 View

What are the precise roles of Machine learning in Drug discovery?

Does anyone know what the end-goal of developing artificial intelligence for aiding the drug discovery process is? How would that theoretically work?

09 April 2019 1,642 10 View

Calculation of drug loading through potentiometric titration?

I am trying to calculate drug loading for an acrylic acid copolymer in which I will be using Poly acrylic acid as a standard, I was just wondering can anyone please tell me that for this study...

31 March 2019 5,776 3 View

Standard curve for absolute telomere measurements by qPCR - > does not work?

12 February 2019 5,436 13 View

Bayesian linear inversion code?

Hi everyone, I am trying to write a Bayesian inversion algorithm for simple linear inversion, but failed to fully understand how to do that. So, can anyone suggest or send a simple...

27 December 2018 5,452 2 View

Bayesian inversion of Ax=b?

I am studying Bayesian inversion and try to write a code for Bayesian inversion and MCMC/RJMCMC. The inversion I intend is simple linear system Ax=b, I have used steepest descent and conjugate...

05 September 2018 4,858 3 View

What are the methods of measuring porosity of inkjet printed ceramic coating on top of porous alumina substrate?

I have 5 sets of coated samples and my intention is to determine the average pore size of the coating for each of them. The sample under examination is a IJP coating of YSZ material on porous...

02 August 2018 6,501 4 View

Does anyone know that can i use sudan black b instead of sudan 4 in En-face staining of whole aorta?

I don't have Sudan 4 hence I want to know I can use Sudan Black instead of Sudan 4

09 June 2018 5,028 3 View

Is it possible to use Hydrochloric acid to regulate the pH for MES buffer?

I have the salt for making the MES buffer ( 2-(N-morpholino)ethanesulfonic acid ) and 0.1M solution of it give me a PH of almost 7 which I have found out it's due to the DI water( I used to make...

25 December 2017 3,338 2 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View