Can i use DTT during a Zn affinity chromatography?

I need to purify His6 tag containing protein and im using a Histrap that is been charged with Zn2+. Can i add DTT to my lysis buffer or the column will not tolerate it when i insert the lysate to...

12 October 2020 5,910 4 View

How can I solve a checkpoint file problem in Gromacs 2018.4?

Hello everyone, I am running a simulation for 500 ns but after 200 ns I get the following error: The input requested 100000000 steps, however the checkpoint file has already reached step...

16 January 2020 454 7 View

How to model Constant-Current Constant-Voltage Charging of Lithium-Ion Battery in Comsol?

I am trying to implement a constant-current constant-voltage charging strategy using the COMSOL standard lithium-ion battery model that comes with the chemical engineering module. The idea is to...

02 December 2019 9,563 3 View

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts)?

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts). Degassing takes place with 5 cycles of 1 minute degassing in vacuum and 1 minute...

18 November 2019 5,423 2 View

SEB or SEE for Jurkat and primary human/mice CD4+ T cell activation?

25 July 2017 338 7 View

How to check the observability of a LPV system?

Consider the discrete system x' = A(t)x + B(t)u y = C(t)x' + D(t)u The matrices A, B, C, D contain time-varying parameters (calculated by a second model) which are given in the initial state, but...

19 May 2017 2,813 1 View

How to detect low amounts of mRNA by qPCR?

I want to detect early expression of IL2 by qRT-PCR. I was not successful using random hexameric primers at the RT step and SYBR for detection. what can I do to improve my sensitivity? What...

01 February 2017 7,322 4 View

How do I calculate sensitivity of android acccelerometers?

Hello, I would like to make a sensitivity experiment on android built-in accelerometers (tri-axonal) an make a statistical analysis (correlation etc) in Matlab. My questions are: 1..What would be...

08 November 2016 9,127 1 View

Any suggestions for efficient Raji cell transfection?

Hi everyone. Has anyone worked with Raji cell transfection? I recently used AMAXA technology to transfect Raji cells with a CRISPR construct that resulted in a very poor efficiency (~1%). When I...

17 May 2016 5,476 4 View

Could Raman spectroscopy reveal the presence of Pt and Ru nanoparticles dispersed on a carbon powder?

09 November 2015 8,352 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View