Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

What is the best way to isolate multiple Salmonella serovars from a single sample?

I am trying to isolate multiple Salmonella serovars from a single food sample. I started by incubating the sample in a non-selective BPW enrichment and from there used multiple selective...

19 January 2021 8,365 4 View

Can someone help me to improve the MCF-10A growth?

18 January 2021 7,640 3 View

Biotech final year project for a bioinformatics beginner?

So my last year project is Drug Efflux Pumps and Persistence in Methicillin Resistant Staphylococcus aureus and we gonna focus on persister cells to study the path way of antimicrobial...

09 December 2020 2,574 2 View

Who can i take a smear from inhibition zone?

I used alcoholic extract of peganum harmala seeds as inhibitor to staphylococcus enterotoxin type A gene (SEA). By using diffusion method I need to know how can i take a smear from zone of...

29 November 2020 3,165 3 View