Has anyone used ChromaClade to visualize amino acid sequence data and phylogenies?

Recently, Christopher Monit, Richard A. Goldstein & Greg J. Towers published an article entitled 'ChromaClade: combined visualisation of phylogenetic and sequence data' in BMC Evolutionary...

17 November 2019 1,637 2 View

Are there any reliable sources for interpreting Transmission Electron Microscope images of Ascomycota hyphae?

I've recently imaged a pathogen in the Phylum Ascomycota and am having difficulty discerning between different organelles and possible endosymbiotic bacteria. I can't seem to find any reliable...

31 July 2019 5,663 2 View

Has anyone researched the psychological motivations of activists?

Activists (for human, animal and conservation causes) who are truly dedicated to their causes tend to sacrifice a great deal: personal safety, material comforts, interpersonal relationships, peace...

06 August 2018 3,620 3 View

Shimadzu HPLC Troubles?

So we are having some issues with a Shimadzu HPLC. The main issue is that we aren't receiving any real time data. In real time we barely get anything on the chromatogram, but if we view a postrun...

05 February 2018 7,368 2 View

Can someone help me with a protocol for mouse skeletal muscle isolation?

Hi Everyone, I've been trying to isolate RNA from mouse skeletal muscles (soleus, gastro, EDL, etc.) using the QIAGEN RNeasy mini kit. However, I've been having issues with low yield of RNA...

19 December 2016 1,475 1 View

Temperature sensor for Eppendorf 5417R refrigerated centrifuge?

Hi, the temperature sensor on my ancient 5417R has finally given up. Might anyone have a spare sensor, or a 5417R lying in disuse that could be scavenged from? Any help would be appreciated as the...

07 December 2016 9,241 2 View

Will freezing crab hemolymph in liquid nitrogen preserve acid-base characteristics?

We need to preserve crab hemolymph to evaluate acid-base characteristics at a later date. Will freezing and holding in liquid nitrogen allow this? Thanks.

21 December 2015 1,197 1 View

Is UV disinfection effective in the interior of pipette tips and plastic tubes?

We want to eliminate potential contamination of plastic tubes and large pipette tips (5 mL). Will UV radiation disinfect the interior of the tubes and tips, passing through the plastic walls? Most...

30 June 2015 4,773 9 View

Could someone provide me with the primer sequences for shrimp gill NKCC symporter?

Has anyone been successful in amplifying the sodium-potassium-two chloride transporter in caridean shrimp gills? Could you provide the primer sequences please.

04 March 2015 2,389 2 View

Has anyone heard of a comparison of relative size of specific brain regions in different mammal species?

09 February 2015 269 5 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View