What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Sample is in a powdered form - how do I prepare for DLS?

I have this question from @Anurag Nath posted on an existing thread: https://www.researchgate.net/post/What_are_some_effective_sample_preparation_techniques_for_DLS_study To avoid confusing the...

06 January 2021 4,856 2 View

Does anyone have an insight into the behavioural response of fishes to moving wind turbine blades as seen through the air-water interface?

I am interested to find out if wind turbines located close to rivers or lakes, or perhaps even in marine locations, are a potential problem for fish. This seems to be a very under-researched area...

04 January 2021 3,468 2 View

Why is my TiO2 sol milky?

@Krunal Dholiya has sent me this question which I post below so he can get the benefit of many experts. Dear, I am preparing a thin film using TiO2 sol but i m fasing problem, with time it is...

23 November 2020 1,188 2 View

Zeta potential and concentration?

I have a question from @Pratima Adhikari that I will post on the open forum so all experts can contribute. Hi Alan, I hope you are fine and healthy. I have a question for you. I am currently...

22 October 2020 3,377 3 View

How to determine the mass of an ore material to be added into a laboratory SAG mill?

Please, I have a mill of volume ~10,000cm3 and I want to calculate the mass of rock sample (-10+5mm) that will occupy 25% of the mill volume. I understand that 25% will be equivalent to 2500cm3....

16 August 2020 5,664 3 View

How to distinguish different phases on an xrd pattern?

In XRD, how do you distinguish among the crystallized phase, amorphous phase, and a mixture of the two? How will the peak look like in a mixture of a crystalline and amorphous material?

05 May 2020 9,707 7 View

Which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes?

which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes? which format is best interleaved or sequential formats? Can you please explain?

22 February 2020 4,878 3 View

Possibility of inculcating INTELLIGENT MANAGEMENT SYSTEM to Higher education system?

The impact of IMS(INTELLIGENT MANAGEMENT SYSTEM) in the industry as well as economy in multiple countries at multilevels had been creating immense impact in the way the whole system was developed...

03 December 2019 4,284 3 View

What can be the possibility of inculcating behavioral finance to the existing studies conducted?

What are the possibilities of incorporating BEHAVIOURAL FINANCE to the existing stream of studies that already gave a clear base to the subject for Phd? What are the possibilities of looking at...

03 December 2019 5,884 11 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

Separation of CaO from Al2O3 and SiO2 in a solid waste, does HCl work?

I have an alkaline solid waste which contains the following components: SiO2, Al2O3, and CaO according to the XRF analysis (as it gives the results as oxides). However, I know from the XRD that it...

28 January 2021 9,469 3 View

How does a pH 13 buffer solution containing NaOH/KCl work?

pH 13 Buffer solution is prepared from a strong base sodium hydroxide and potassium chloride salt. However a buffer contain a weak base or weak acid with their salts. What is the purpose of the...

26 January 2021 9,154 1 View

Can someone help me to improve the MCF-10A growth?

18 January 2021 7,640 3 View

Can I use hcl to adjust ph rather than glacial acetic acid?

In alkaline lysis solution3 acetic acid should be used to adjust pH according to protocol. Can I use hcl instead of glacial acetic acid? Will there be any change in composition of solution?

08 January 2021 7,764 3 View

Is there a way to improve the stability of the calcium alginate beads in alkaline pH?

I am working on heavy metal bioremediation using bacterial isolates. I have immobilized bacteria in 2% sodium alginate then cross-linked in 0.2M calcium chloride for 30 min. I have successfully...

04 January 2021 9,094 4 View