To prepare 1 liter stock 1 solution for MS medium, following constituents were added: (NH4)2 SO4 33g; KNO3 38g; CaCl2 0.88g, MgSO4 7.4g, KH2PO4 3.4g. Here ammonium nitrate NH4NO3 is replaced with ammonium sulphate (NH4)2 SO4. on doing so, it was observed that there is formation of precipitate into the stock immediately. looking forward, for the replacement of ammonium nitrate as well as what precaution, must be taken to avoid precipitate formation on adding ammonium sulphate while preparing stock 1 solution for MS media.
Have some idea here too..
http://www.fgsc.net/neurosporaprotocols/How%20to%20choose%20and%20prepare%20media.pdf
Thank you https://www.researchgate.net/deref/http%3A%2F%2Fwww.fgsc.net%2Fneurosporaprotocols%2FHow%2520to%2520choose%2520and%2520prepare%2520media.pdf
i have gone through the link and i found out that (NH4)H2PO4 can be used as alternative of ammonium nitrate, but here the complication is, at what concentration it should be used so that the ratio of NH4, NO3 , and PO4-2 wont hinder in the MS media composition.
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