Does anyone have an RNA preservation protocol that yielded good next generation transcriptome sequencing results from preservation of plant organs in RNAlater (or another preservative) under field conditions (no refrigeration for >1 week)?
This is not exactly a recommendation, but have you seen this? http://www.bioone.org/doi/abs/10.3732/apps.1300070
I have used RNA later for RT PCR, I stored my tissue for couple of weeks in -20 in RNA later
Yes, I always kept RNA samples at -20 degree C. Probably it will work for a long time.
I understand- When out in the field, try to put your samples in a cooler if you can (and refrigerate when you can). Immediately put in a 4C refrigerator when you get back over night. Drain the samples and freeze at -80C until you are ready to use. Depending on your samples, extract as you normally would. (I work with weirdos so kits don't always work for me.) You'll tend to see that the 260/230 ratios will be low- this happens with all of my RNALater samples. Sometimes LiCl precipitations help. (If you are lucky enough to get enough RNA that you won't lose it all.) I've continued on without the 'cleanest' looking RNA for next gen transcriptome sequencing (Illumina) and things have worked out pretty good.
Good luck!
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