Im using 2,4 D , Kinetin, BAP for callus induction and somatic embryo genesis of an woody plant. I"m using the above hormones above. Is those hormones are autoclavable?
hi
Sterile technique - Molecular weight - Abbreviated chemical name
CA/F 175.2 IAA
CA/F 203.2 IBA
CA/F 241.3 KIBA
CA 186.2 NAA
CA 221 2,4-D
CA/F 225.3 BA
F 247.7 4CPPU
F 212.3 DPU
CA/F 203.3 2ip
CA 215.2 Kinetin
CA 220.2 TDZ
CA/F 219.2 Zeatin
CA/F 346.4 GA3
CA/F 384.5 KGA3
CA/F 264.3 ABA
CA/F: Autoclavable with other components of the culture medium may reduce the activity of some components
CA: Autoclavable with other components of the culture medium
F: Cold filtration
some of the hormones are co - autoclavable with other media components
2,4-Dichlorophenoxyacetic acid,
Indole-3-acetic acid Free acid (IAA)
6-Benzylaminopurine (BA)
Kinetin
all are co - autoclavable,
however, some loss of activity may occur. This can be compensated for by increasing component concentration.
best option is that the Component may be filter sterilized.
all the best
Yes
2,4-D, NAA, IBA,IAA, BA, TDZ , BAP co - autoclavable with other media components and GA non-autoclavable and must be filter sterilized.
but however, some loss of activity may occur. for example, IAA after 20 min autoclave 40% loss activity. but 2,4-D, NAA, IBA are resistant.
Yes u can autoclave the Plant Growth Regulator,,GR is stable in autoclave time and lose some action then compare to plant hormones
Filter sterilization is best & safer option compared to autoclaving the hormones
An interesting related point that I did not know before (perhaps the rest of you do): some PLANT hormones may be useful in treatment of cancer in HUMANS.
See: https://en.wikipedia.org/wiki/Plant_hormones#Potential_medical_applications
We are using BAP and IBA in our propagation medium for elms. All medium ingredients were autoclaved together. We had never any problems with that method.
hi
Sterile technique - Molecular weight - Abbreviated chemical name
CA/F 175.2 IAA
CA/F 203.2 IBA
CA/F 241.3 KIBA
CA 186.2 NAA
CA 221 2,4-D
CA/F 225.3 BA
F 247.7 4CPPU
F 212.3 DPU
CA/F 203.3 2ip
CA 215.2 Kinetin
CA 220.2 TDZ
CA/F 219.2 Zeatin
CA/F 346.4 GA3
CA/F 384.5 KGA3
CA/F 264.3 ABA
CA/F: Autoclavable with other components of the culture medium may reduce the activity of some components
CA: Autoclavable with other components of the culture medium
F: Cold filtration
Generally synthetic harmones are autoclavable. 2,4-D and BAP are also. Kinetin is natural and it is heat liable. you can autocalve but u have to add more. Best option is to filter sterlize all these harmone.
Many of the plant hormones are simple organic molecules which can be autoclaved without losing any potency unlike the protein hormones
The hormone 2,4-D, Kinetin and BAP are autoclavable and GA and Zeatins are temperature sensitive which have to be filter sterilized.
Dear researcher
yes. These hormones are autoclaveable, you can also for more information use attached file.
Yes hormones like NAA, BAP, 2,4-D are autoclavable. But filter sterilization is needed for some hormones like IAA, TDZ, GA.
I agree with u dr. Ravinder . Regarding the GA u can dissolve it in absolute alcohol, than u can add to medium after autoclaving. But keep in mind this thing must be achieved under aseptic condition. IAA is autoclavable but it can be sterilized by filtration
I would use 95% etoh, NOT absolute as it has an organic solvent added (generally Benzene) to remove/capture H2O and is therefore poisonous and may cause issues with tissue culture (remember we use only 70% etoh to sterilize). And YES, you can autoclave the synthetics, but sterile filtration removes "floaters" and is quick and easy. :)
Dear David Warren
A millipore filter (0.22 µm) can be used. Empty glass is sterilized in an autoclave before filter sterilization. prepare a required aqueous stock solution of GA3 (mg of GA3/ml of D.water). sterilize the medium by autoclave,then cooled to 50-60 °C before adding GA3 under aseptic conditions.....good luck...............regards
How long do the hormones remain in the culture medium (solid medium)?
sir i am using NAA and GA3 for exogenous application how i can dissolve them i tried to dissolve in ethanol and then in distilled water but precipitates are formed
Yes why not?
There are exceptions like the GA3 that needs to be filtered
One can dissolve hormones in DMSO and add to stelie medium after autoclaving. Only one pre-caution_ final DMSO concentraztion must be less than 0,5 µl/ml.
But all hormines can be dissolved in DMSO at high concentration.
My best wishes.
Dear IShtiaq Ahmad, to use NAA and GA3, you can dissolve them in sodium hydroxyde (NaOH 1M).
How many times autoclave after adding BAP and IAA into the MS medium?
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