I have FITC labeled proteins, and I would like to detect its fluorescence after running proteins western blot. Does fluorescence quench in WB condition? so I can detect FITC labeling (without...
27 May 2024 4,087 3 View
hi there, I'm looking for assessing the importance of different features within a mixed linear model, and to be able to tell statistically (i.e. to be able to talk about significant difference)...
07 May 2024 8,177 6 View
Edit: image uploaded I've blotted for beta-actin on cell culture protein lysates many times but the beta-actin detection range was not between 37 - 50 kDa range but 25 - 37 kDa range each time....
09 September 2021 4,074 4 View
I have been working on astrocyte from primary glial cell culture and I want to observe calcium influx after several treatment. I know once Fura-2 AM in the cell, intrinsic esterases hydrolyze the...
18 February 2021 754 3 View
Please provide me simulation models in MATLAB for artificial pancreas,Glucose insulin modelling Also some tutorials or videos or files to learn development of Artificial pancreas simulation
30 May 2019 3,252 3 View
I have a list of protein IDs, from NCBI. The names are unique for a specific protein in a specific genome. I want to find the genome from which they come from. I could do this manually using the...
09 March 2019 3,543 1 View
Hey all, I'm using the Herek ATLG. Attitudes toward homosexuality does not distributed normally (positive skew). I have two independent variables with 2 levels each and I want to know the...
28 January 2019 405 3 View
I want to choose one of several options for 16S metagenomics analysis. I want to check if several specific sequences will come up in the PCR. Is there an easy way to do it? the reference...
28 April 2018 7,211 2 View
I have a tank of lube oil which is not insulated and top of tank is a compartment, in other words, tank is the bottom of the auxiliary compartment. The volume of tank is 25.000 liters. Because we...
27 March 2018 1,561 5 View
Hi, I have a problem to get a good RIN value from my RNA isolation for mirna research, I did it with qiagen mirneasymini kit, and trizol method (with different trizols for example I used qiazol...
07 June 2015 1,742 11 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View
