Please , I need this antibody for my research
Phospho-KIN10 (Thr175) Antibody or Phospho-SnRK1alpha1 (Thr175) Antibody for detection of endogenous SNF1-related protein kinase catalytic subunit alpha KIN10 (SnRK1alpha1) protein only when phosphorylated at threonine 175 in Broccoli, scientifically known as Brassica oleracea var. italica.
Zein Al-Abideen Douba
Check supplier specificity: Many commercial antibodies (e.g., from Agrisera, Abcam, or Thermo Fisher) provide a list of tested species. If broccoli is not listed, you may need to test cross-reactivity experimentally. Sequence alignment: Compare the amino acid sequence around Thr175 of broccoli SnRK1α1 with Arabidopsis or other species for which the antibody is validated. High conservation increases the likelihood of successful detection. Experimental validation: Perform a Western blot with total protein extract from broccoli and include positive controls (e.g., Arabidopsis) to confirm specificity. Alternative approaches: If no antibody works reliably, consider using mass spectrometry or designing a custom antibody specific for the broccoli SnRK1α1 phosphorylation site
Hello,
Phospho-KIN10 (Thr175) or Phospho-SnRK1alpha1 (Thr175) antibodies are generally developed for model plants such as Arabidopsis thaliana, and their cross-reactivity with broccoli (Brassica oleracea var. italica) is not always guaranteed. Here are some suggestions:
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